To this end we used GeneSpring software, with summarization algorithm RMA16, filtered on raw expression minimum of 150 in at least three samples, performed ANOVA P,0.05, and required minimum three fold enrichment in adult podocytes compared to total cortex, giving a total of 436 probesets. In a second strategy we searched for genes that were active in the adult podocyte compared to the developmentally early E13.5 podocyte. The underlying hypothesis is that the E13.5 podocyte is largely undifferentiated, and as the differentiation program ensues the set of genes that distinguish podocytes from other cells will become active. A similar analysis of the data was performed, Abmole Company Masitinib requiring a minimum three fold enrichment, this time between adult podocytes and E13.5 podocytes. This approach yielded 739 probesets, with the two strategies having an overlapping set of 281 probesets. Each strategy alone is imperfect. Some genes that are of key importance in the podocyte might also show significant expression in other regions of the kidney cortex. These genes would be unfortunately subtracted in the screen looking for enrichment compared to kidney cortex. And, some genes that are specific markers of the podocyte, such as MafB, are already active at E13.5. These genes would be eliminated by a screen that looks only for genes with transcripts enriched in the adult podocyte compared to E13.5. Nevertheless it is reassuring that the two strategies yield similar sets of genes, with about 2/3 of the 436 probesets found by comparison to kidney cortex also identified by the screen comparing adult to embryonic. We first cast a wide net, looking to create a comprehensive catalog of genes whose expression defines the adult podocyte. We therefore combined the lists made with the two screening strategies creating a set of 894 probesets. A heatmap provides a visual representation of the relative abundances of transcripts of adult podocytes compared to E13.5, and E15.5 podocytes, as well as total kidney cortex .