For this purpose, we considered the localization of GFP-tagged DmRad9A in okr mutants, a Rad54-like protein, a double-strand DNA breaks repair enzyme, and in flies expressing a non-phosphorylatable form of BAF. DmRad9A localization is not affected in the background of over-expression of BAF3A. Were DmRad9A involved in the physical connection between the chromosomes and the oocyte nuclear envelope, we would have expected to get results similar to what was shown for otefin. In the wild type oocyte nucleus, otefin is found at the nuclear membrane. However, upon over-expression of BAF3A, otefin accumulated in a region of the nuclear envelope in close contact with meiotic chromosomes and was absent from other region of the oocyte nuclear membrane. On the other hand, persistence of DSBs, as observed in okr mutants, dramatically affected DmRad9A oocyte nuclear membrane localization. Thus, the displacement of DmRad9A from the oocyte nuclear membrane due to activation of a meiotic checkpoint is probably part of the oocyte response to DSBs, rather than reflecting a step in the process of attachment of the meiotic chromosome to the nuclear membrane. Ischaemia/reperfusion injury is an important complication of acute arterial occlusion and subsequent recanalisation; for example, following acute myocardial infarction, coronary artery recanalisation by thrombolytic therapy or percutaneous coronary intervention is used therapeutically in an attempt to minimize the infarct area. However the reoxygenation of the ischaemic heart leads to an area of myocardial loss of function. Many signaling molecules have been postulated to contribute to ischaemia-reperfusion injury including both reactive oxygen species and nitric oxide. NO, acting as a signaling molecule, plays a major regulatory role in several aspects of cellular function; for example, it causes vasodilatation, inhibits platelet function and leukocyte-endothelial interaction and modulates neurotransmission. NO can also exert a potent anti-inflammatory effect, by suppressing adhesion molecule expression and cytokine release. Hypoxia-inducible factor-1, is a transcription factor expressed in response to a decrease in the partial pressure of cellular oxygen. It is a heterodimer composed of a and b subunits. HIF-1a is stable in physiological condition and exquisitely sensitive to the onset of cellular hypoxic Compound Library conditions, while HIF-1b is constitutive and not sensitive to hypoxia. HIF-dependent genes, such as vascular endothelial growth factor, are important in I/R injury via regulating collateral vessel development. Tetrahydroprotoberberines are a series of alkaloids, isolated from a Chinese analgesic medicine, called Corydalis yanhusuo W. T. Wang. l-Tetrahydropalmatine, one of its main active ingredients, has been demonstrated to have potent analgesic effects and has been in use in Chinese clinical practice for this purpose for many years. Its chemical structure is shown in Figure 1. Studies have shown that giving l-THP before ischaemia can protect against acute global cerebral ischaemia-reperfusion.