As part of the EU-funded joint research project ��MicroBioMed��,we aimed to identify novel putative protein biomarkers for later integration into a micro-fluidic chip system suitable for early cancer detection or disease monitoring. In the Donepezil hydrochloride present study, AGR2 andAGR3 were identified for the first time as putative serum protein biomarkers in breast cancer. Moreover, AGR3 was found to be an independent prognostic factor of unfavourable prognosis in lower grade breast cancer cases, indicating a tumour-promoting function in well to moderately differentiated breast carcinomas. IM-12 neuronal development is a tightly regulated multi-step process. Neural stem cells proliferate, differentiate and mature to give rise to the neuronal morphology and fully functional neurons. Timely maturation of neurons, characterized by axonal and dendritic outgrowth, synaptogenesis, synaptic and neuronal pruning, modulation of neurotransmitter sensitivities and myelination, determines neuronal connections with extraordinary precision. These culminate into large, integrated networks of synapses with specific functions in the brain. Gene expression throughout the neuronal maturation process is intricately regulated by distinct temporal and spatial expression of specific non-coding RNAs, namely microRNAs and long ncRNAs. miRNAs, the most well characterized ncRNAs, are short endogenous molecules, approximately 22 nucleotides in length. In general, these small ncRNAs interact with their target mRNAs by complementary binding to bring about transcriptional and translational regulation. Brain-specific and brain-enriched miRNAs, miR-124 and miR134, are vital regulators of neuronal functions associated with neurogenesis and synaptic plasticity respectively. LncRNAs, on the other hand, are transcripts longer than 200 nucleotides. These RNA molecules coordinate gene expression through epigenetic modification, mRNA splicing, control of transcription or translation and genomic imprinting. LncRNAs have been shown to play a role in embryogenesis and development of the central nervous system.