Among these solutions, PBS is most frequently selected as the vehicle for NSC suspension and transplantation, while ACSF is generally employed in electrophysiological assays. Because of the physiological compatibility, these harvesting media are barely reported to have adverse effects on cells. Currently, almost no data exists regarding the effects of these solutions on NSCs and their post-mitotic progeny. Additionally, there is still no consensus on the optimal treatment conditions. However, due to the lack of trophic support provided by harvesting media and to variations in the treatment conditions, the characteristics and the repair potential of NSCs can vary. In this study, we PHA-848125 explored the effects of different harvesting media and different treatment durations on the viability, proliferation, cell cycle progression, differentiation, apoptosis, necrosis and repair potential of NSCs. We sought to reduce the adverse effects and to maximize transplanted cell survival and functional recovery. The viability and proliferation of NSCs are key factors in successful NSC transplantation. According to the criteria of the FDA, transplanted cells should maintain viability and proliferation levels above the permissible limit. Our data indicate that following prolonged treatment, the viability and proliferation of NSCs gradually are deteriorated. The viability and proliferation of NSCs are influenced by multiple exogenous instructive factors, such as temperature, osmolarity, pH, and nutrients. In the present study, we employed the harvesting media and the PCM with the same temperature and osmotic Enalapril Maleate pressure. Therefore, the deterioration of the viability and proliferation of NSCs is not caused by the temperature or osmolarity of the harvesting media but likely associated with pH alteration and a lack of nutrients. The experimental harvesting media do not contain any growth factors and only provided short-term survival of NSCs.Deficiency or exhaustion of exogenous instructive factors or lack of pH buffer system may greatly inhibit the viability and proliferation of cells in vitro, while a greater number of NSCs survive in the media containing sufficient nutrients, powerful buffer system and essential growth factors.