The number of Amikacin hydrate literatures which tree shrews are used as an experimental animal model in amount of disease is increasing, because of their well-developed visual system, higher-ratio of brain to body mass, or infection of speciesespecially pathogens, such as hepatitis virus and influenza virus. Currently, tree shrew models are mainly used for research into the nervous, visual systems, and viral infection disease. However, only a few tree shrew genes have the full-length sequences. In the Genbank database, there are only around 200 molecules with coding information. Moreover, there are only a few antibodies and detecting kits available for studying tree shrew. It is difficult to detect and characterize gene and protein expression profile without Povidone iodine sequence information and species-especially antibodies. Due to those reasons, it is narrow the wide application of tree shrew as a model for diseases’ mechanism research. Housekeeping gene is a group of typically constitutive genes that are required for the maintenance of basic cellular function. Some housekeeping genes are expressed at relatively constant levels in most physiological situations; others may vary according to experimental conditions. Therefore, housekeeping genes are generally used as internal reference to normalize target genes in many examine methods, such as quantitative real-time reverse transcription polymerase chain reaction, western immunoblotting, immunohistochemistry, and so on. An ideal housekeeping gene as internal reference should be widely stably expressed within all cells of an organism under normal and pathophysiological conditions. To examines of human samples, some housekeeping genes are frequently used as internal reference, which also named endogenous control genes, such as Glyceraldehyde-3-Phosphate Dehydrogenase, b-actin, 18S ribosomal RNA, heat-shock protein-90, b-2-microglobulin, b-tubulin and others. However, for tree shrew, most of their sequences are still unknown. GAPDH playing an important role in both of glycolysis and nuclear functions, respectively, given that is has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase. GADPH also is an important enzyme in the process of glycolysis and gluconeogenesis, whose cycles occur in the cytoplasm. It is responsible for catalyzing the reversible conversion of glyceraldehyde 3-phosphateand inorganic phosphate into 1,3-bisphosphoglyceratewith a three-step reaction. Moverover, GAPDH has been implicated it is involved in several non-metabolic processes, including transcription activation, ER to Golgi vesicle shuttling, initiation of apoptosis, and axoplasmic transport, or fast axonal. Thereby, GAPDH was considered it is experssed in every cell, tissue, and organ. It is one of “housekeeping” molecules that were used as an internal reference to correct the potential error of RNA/cDNA loading, and variation of reverse transcription efficiency. GAPDH is widely considered as a molecule that is expressed at relatively constant levels in most situations. However, some studies disagreed to this. In this study, we identified full-length cDNA sequence of tree shrew GAPDHgene. Using this sequence, we construct the genetic family tree and study the GAPDH expression profiles in tree shrew. These studies provide the novel genetic and proteomic knowledge of tree shrew, and the strong evidence that tree shrew can be a potential animal model to study human disorders.